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e coli bl21 de3 plyss  (Thermo Fisher)


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    Structured Review

    Thermo Fisher e coli bl21 de3 plyss
    E Coli Bl21 De3 Plyss, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e%2E+coli+bl21(de3/Ampicillin/pm42300662-27-0-3
    Average 99 stars, based on 1 article reviews
    e coli bl21 de3 plyss - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Cell Culture:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    SDS Page:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Affinity Purification:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Expressing:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Solubility:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Activation Assay:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Lysis:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Plasmid Preparation:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Control:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Standard Deviation:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Concentration Assay:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Comparison:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Cryo-Electron Microscopy:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Residue:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Generated:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Amplification:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Construct:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Clone Assay:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Purification:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    In Vitro:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Ex Vivo:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Recombinant:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Methylation:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Incubation:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Transfection:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Immunoprecipitation:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Isolation:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Protein Purification:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Staining:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Migration:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Molecular Weight:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Virus:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Modification:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Protease Inhibitor:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Fluorescence:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Oligonucleotide Synthesis:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Software:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Variant Assay:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).

    Sequencing:

    Article Title: Peptide vaccines against interleukin-31
    Article Snippet: Sequence confirmed plasmids were used to transform E. coli BL21 DE3 (Invitrogen Corp., Carlsbad, Calif.) and subsequent protein expression carried out.

    Article Title: Evolution of PqsE as a Pseudomonas aeruginosa -specific regulator of LuxR-type receptors: insights from Pseudomonas and Burkholderia
    Article Snippet: E. coli BL21 DE3 (Invitrogen) transformed with pET-DUET containing RhlR or CepR were cultured from glycerol stocks in 3 mL of LB + ampicillin at 37 °C with shaking at 200 rpm.

    Article Title: Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation
    Article Snippet: E. coli BL21 DE3 , Thermo Fisher Scientific , Cat# EC0114.

    Article Title: Alpha-hemolysin variants with altered characteristics
    Article Snippet: E. coli BL21 DE3 (from Life Technologies) cells were transformed with the expression vector comprising the DNA encoding the wild-type or variant α-hemolysin using techniques well-known in the art.

    Article Title: Impaired cAMP processivity by phosphodiesterase-protein kinase A complexes in acrodysostosis
    Article Snippet: The catalytic domain of wild-type PDE8A1 (henceforth referred to as PDE8 WT) and PDE8 T690P constructs were cloned into pET-Duet1 and overexpressed in E. coli BL21 DE3 (Thermo Scientific, United States) by induction at OD 600 ∼0.6 with 0.5 mM IPTG.

    Article Title: The phycobilisome linker protein ApcG interacts with photosystem II and regulates energy transfer to photosystem I in Synechocystis sp. PCC 6803
    Article Snippet: Plasmids carrying the truncated form of ApcG for pull-down experiments (pREC54, pREC55 and pREC56) were transformed into E. coli BL21 DE3 (Invitrogen, Carlsbad, CA, USA).

    Article Title: Staphylococcus aureus-specific skin resident memory T cells protect against bacteria colonization but exacerbate atopic dermatitis-like flares in mice.
    Article Snippet: Camille Braun, MD/PhD, Cédric Badiou, MSc, Aurélie Guironnet-Paquet, MSc, Masashi Iwata, PhD, Vanina Lenief, MSc, Amandine Mosnier, MSc, Charlotte Beauclair, MSc, Emilie Renucci, MSc, Pauline Bouschon, MD, Roxane Cuzin, MSc, Yoann Briend, MSc, Vijaykumar Patra, PhD, Sabine Patot, PhD, Tiffany C. Scharschmidt, PhD, Willem van Wamel, PhD, Nicole Lemmens, PhD, Saeko Nakajima, PhD, François Vandenesh, MD/PhD, Jean-François Nicolas, MD/PhD, Gérard Lina, MD/PhD, Audrey Nosbaum, MD/PhD, Marc Vocanson, PhD

    Article Title: Encapsulation of Select Violacein Pathway Enzymes in the 1,2-Propanediol Utilization Bacterial Microcompartment to Divert Pathway Flux
    Article Snippet: The E. coli BL21 DE3 (Life Technologies) cells were prepared into cell-free extracts via growth, harvest, lysis, and preparation as previously described ( , ).



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    Image Search Results


    Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).

    Journal: Frontiers in Veterinary Science

    Article Title: Construction and immunogenicity evaluation of a bivalent nanoparticle based on mi3 displaying porcine circovirus type 2 and type 3 capsid proteins

    doi: 10.3389/fvets.2026.1862938

    Figure Lengend Snippet: Construction of engineered E. coli strains and protein purification. (A) PCR identification of positive E. coli clones. Lanes 1–3 correspond to BL21-pET28-ST-PCV2 Cap, BL21-pET28-ST-PCV3 Cap, and BL21-pET28-SC-mi3, respectively. (B–D) Expression and purification of recombinant proteins ST-PCV2 Cap, ST-PCV3 Cap, and SC-mi3, respectively. Lanes 1–6 represent whole bacterial lysate, pellet after sonication, supernatant after sonication, Ni column flow-through, wash fraction, and purified protein, respectively. (E) Western blot validation of antigen proteins. Lanes 1, 2 correspond to ST-PCV2 Cap and ST-PCV3 Cap, respectively. (F) Endotoxin removal from recombinant protein solutions. Color intensity represents endotoxin content; numbers indicate endotoxin content (EU/mL).

    Article Snippet: The pET28a(+) plasmid for recombinant protein expression and BL21(DE3) E. coli competent cells were purchased from Sangon Biotech (Shanghai) Co., Ltd.

    Techniques: Protein Purification, Clone Assay, Expressing, Purification, Recombinant, Sonication, Western Blot, Biomarker Discovery